Where can I find the protein concentration?

The protein concentration of each product is listed on the Certificate of Analysis. This value is determined for each individual lot and reflects the actual concentration (mg/mL) of the material you received.

Where can I find the recommended storage conditions?

Storage conditions are listed on both the product datasheet and the Certificate of Analysis. These recommendations are based on internal stability assessments and are intended to maintain protein integrity and activity.

Where can I find reconstitution instructions for lyophilized products?

If your product is supplied lyophilized, recommended reconstitution instructions are provided on the product datasheet. These instructions outline the suggested solvent and handling tips to ensure optimal recovery and stability.

How should I store my product after reconstitution?

Unless otherwise stated on the datasheet or COA, we recommend aliquoting reconstituted material and storing at –80 °C to avoid repeated freeze–thaw cycles, which may impact protein stability and activity.

Elastase

Name: Elastase
SKU: 16-14-051200-50UG
Availability: InStock

Product Description

Role of protein in biology

Neutrophil elastase (NE), a serine protease stored in azurophilic granules of neutrophils, plays critical roles in host defense and tissue remodeling. As a member of the chymotrypsin superfamily, NE hydrolyzes elastin, collagen, and proteoglycans through its catalytic triad (His-Asp-Ser), enabling pathogen clearance via microbial membrane disruption. Beyond direct bactericidal activity, NE modulates immune responses by activating matrix metalloproteinases (MMPs), inducing pro-inflammatory cytokines (TNF-α, IL-1β, IL-8), and promoting neutrophil extracellular trap (NET) formation. However, unchecked NE activity drives extracellular matrix degradation, contributing to chronic inflammatory and degenerative diseases.

Common research uses

NE dysregulation is central to pulmonary emphysema, particularly in α1-antitrypsin deficiency, where insufficient protease inhibition leads to alveolar destruction. In chronic obstructive pulmonary disease (COPD), NE excess exacerbates airway inflammation and mucus hypersecretion, perpetuating lung parenchymal damage. NE also directly injures cartilage in rheumatoid arthritis by degrading collagen and activating fibroblast-like synoviocytes, amplifying joint inflammation. Additionally, elevated NE levels correlate with cystic fibrosis progression, acute respiratory distress syndrome (ARDS), and severe COVID-19, where it enhances viral entry and cytokine storms. Balancing NE’s protective and pathogenic roles remains a clinical challenge, necessitating therapies that preserve antimicrobial function while preventing tissue destruction.

Common uses include IVD Assay standards, calibrators and controls, proteolytic enzyme studies, pulmonary reseach, biotherapeutics.