Neutrophil lactoferrin is a multifunctional glycoprotein stored in the secondary granules of polymorphonuclear neutrophils and released during immune responses. In addition to its presence in exocrine secretions such as saliva, tears, and milk, neutrophil-derived lactoferrin plays a pivotal role in the body’s innate defense mechanisms. Upon release, it binds free iron with high affinity, limiting iron availability to invading bacteria and thereby exerting potent bacteriostatic and bactericidal effects. This iron-sequestration not only inhibits microbial growth but also helps modulate inflammatory responses at sites of infection. Furthermore, neutrophil lactoferrin regulates intestinal iron absorption, contributing to iron homeostasis and preventing iron overload or deficiency.