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The protein concentration of each product is listed on the Certificate of Analysis. This value is determined for each individual lot and reflects the actual concentration (mg/mL) of the material you received.

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Where can I find the final buffer composition?

The final buffer formulation is provided on the Certificate of Analysis. This includes buffer components, pH, and whether the product was supplied as a liquid or lyophilized powder.

Immunoglobulin G1, Fab Fragment, Myeloma

Name: Immunoglobulin G1, Fab Fragment, Myeloma
SKU: 16-16-090707-1M-Fab-1MG
Availability: InStock

IgG1 Fab

The monovalent antigen binding fragment of IgG generated by proteolytic digestion. IgG Fab retains the antigen specific variable domains, while lacking the immune activating Fc regions.

Purity: ≥95% (SDS-PAGE)

Source: Human Myeloma Plasma

Molecular Weight: 50 kDa

Form: Frozen

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Product Description

Role of protein in biology

The IgG Fab (Fragment antigen-binding) fragment is a monovalent proteolytic product of Immunoglobulin G. Each Fab fragment retains the variable domains (VH and VL) responsible for antigen specificity while lacking the Fc-mediated effector functions, enabling targeted antigen neutralization without immune activation. This structural configuration prevents cross-linking-induced precipitation or cellular "patching/capping," making Fab fragments ideal for precise antigen targeting in solution or on cell surfaces.

Common research uses

Diagnostically, Fab fragments enable multiplex immunoassays by preventing Fc-mediated cross-reactivity, facilitating simultaneous detection of multiple antigens in flow cytometry and immunohistochemistry. Their monovalent nature ensures minimal background noise in assays requiring high specificity. Emerging applications include autoimmune disease management, where Fab fragments neutralize autoantibodies while circumventing complement activation. By combining targeted binding with reduced immunogenicity, IgG Fab fragments represent a versatile tool in both clinical and research settings.

Common uses include therapeutics research, immune system research, flow cytometry assays, immunohistochemistry assays.