Where can I find the protein concentration?

The protein concentration of each product is listed on the Certificate of Analysis. This value is determined for each individual lot and reflects the actual concentration (mg/mL) of the material you received.

Where can I find the recommended storage conditions?

Storage conditions are listed on both the product datasheet and the Certificate of Analysis. These recommendations are based on internal stability assessments and are intended to maintain protein integrity and activity.

What should I do if my product arrives warm, partially thawed, or damaged?

Please contact Athens Bioscience with your order number and lot number. Our technical support team will assist you promptly.

Where can I find the final buffer composition?

The final buffer formulation is provided on the Certificate of Analysis. This includes buffer components, pH, and whether the product was supplied as a liquid or lyophilized powder.

Immunoglobulin G, Fab'2 Fragment

Name: Immunoglobulin G, Fab'2 Fragment
SKU: 16-16-090707-FAB'2-1MG
Availability: InStock

IgG Fab'2

The bivalent antigen binding fragment of IgG from proteolytic digestion. The connection of the two antigen binding regions by disulfide bonds is maintained.

Purity: ≥95% (SDS-PAGE)

Source: Human Plasma

Molecular Weight: 110 kDa

Form: Frozen

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Product Description

Role of protein in biology

The IgG F(ab')₂ fragment is a bivalent, proteolytic product of Immunoglobulin G, retaining two antigen-binding (Fab') regions connected by disulfide bonds in the hinge region while excluding the Fc domain2. This structural configuration preserves avidity through dual antigen engagement, offering a higher association constant compared to monovalent Fab fragments-particularly advantageous for low-affinity antibodies requiring enhanced binding stability. The absence of the Fc region eliminates interactions with Fc receptors and complement proteins, minimizing nonspecific immune activation and background noise in diagnostic assays3.

Common research uses

Therapeutically, F(ab')₂ fragments are pivotal in antivenom formulations where rapid toxin neutralization without Fc-mediated inflammation reduces adverse reactions and mitigates late coagulopathy in rattlesnake envenomation. In oncology, their smaller size enhances tumor penetration, while faster blood clearance improves imaging signal-to-noise ratios in radiolabeled diagnostics immunologically, Diagnostically, F(ab')₂ fragments enable precise antigen detection in flow cytometry and immunohistochemistry by avoiding Fc receptor cross-reactivity. Their divalent binding also stabilizes antigen-antibody complexes in immunoassays, ensuring high specificity for low-abundance targets. Emerging applications include autoimmune disease management, where F(ab')₂ fragments neutralize autoantibodies while circumventing complement activation. By combining targeted avidity with reduced immunogenicity, IgG F(ab')₂ fragments bridge therapeutic efficacy and diagnostic precision across diverse clinical contexts.

Common uses include therapeutics research, immune system research, flow cytometry assays, immunohistochemistry assays.